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cd163 fitc  (Bioss)


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    Structured Review

    Bioss cd163 fitc
    M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while <t>CD163,</t> ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.
    Cd163 Fitc, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+conjugated+cd163/CD163+M130+Polyclonal+Antibody%2C+FITC+Conjugated/pmc12019731-134-4-7
    Average 91 stars, based on 8 article reviews
    cd163 fitc - by Bioz Stars, 2026-10
    91/100 stars

    Images

    1) Product Images from "Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages"

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    Journal: ACS Omega

    doi: 10.1021/acsomega.4c10118

    M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while CD163, ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.
    Figure Legend Snippet: M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while CD163, ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.

    Techniques Used: Flow Cytometry, Standard Deviation

    Liver expression of AKT (a–c), TGFb (d–f), CD163 (g–i), CD86 (j–l), NFkB (m–o), IL-10 (p–r), and Pi3K (s–u) in Wistar rats submitted to alcoholic liver injury.
    Figure Legend Snippet: Liver expression of AKT (a–c), TGFb (d–f), CD163 (g–i), CD86 (j–l), NFkB (m–o), IL-10 (p–r), and Pi3K (s–u) in Wistar rats submitted to alcoholic liver injury.

    Techniques Used: Expressing

    Liver expression of AKT (a), TGFb (b), CD163 (c), CD86 (d), NFkB (e), IL-10 (f), and Pi3K (g). All data are presented as mean ± SD. Fluorescence intensity data was generated by Zeiss Zen Lite software for each picture taken (10 images per subject, 5 animals per group, 400× magnification). Statistically significant differences are indicated as follows: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc correction for multiple comparisons.
    Figure Legend Snippet: Liver expression of AKT (a), TGFb (b), CD163 (c), CD86 (d), NFkB (e), IL-10 (f), and Pi3K (g). All data are presented as mean ± SD. Fluorescence intensity data was generated by Zeiss Zen Lite software for each picture taken (10 images per subject, 5 animals per group, 400× magnification). Statistically significant differences are indicated as follows: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc correction for multiple comparisons.

    Techniques Used: Expressing, Fluorescence, Generated, Software

    Comparison between M1 (CD86) and M2 (CD163) immunofluorescence labeling in Wistar rats submitted to alcoholic liver injury. All data are presented as mean ± SD. Fluorescence intensity measurements were obtained using Zeiss Zen Lite software, with 10 images captured per subject, 5 animals per group, at 400× magnification. Statistically significant differences are denoted as * p < 0.05 and ** p < 0.005; (ns) indicates no significant difference. Data were analyzed using two-way ANOVA with Sidak’s post hoc correction.
    Figure Legend Snippet: Comparison between M1 (CD86) and M2 (CD163) immunofluorescence labeling in Wistar rats submitted to alcoholic liver injury. All data are presented as mean ± SD. Fluorescence intensity measurements were obtained using Zeiss Zen Lite software, with 10 images captured per subject, 5 animals per group, at 400× magnification. Statistically significant differences are denoted as * p < 0.05 and ** p < 0.005; (ns) indicates no significant difference. Data were analyzed using two-way ANOVA with Sidak’s post hoc correction.

    Techniques Used: Comparison, Immunofluorescence, Labeling, Fluorescence, Software

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    Incubation:

    Article Title: The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage in costal cartilage defects.
    Article Snippet: After harvesting multiple costal cartilages, the local defect disrupts the integrity of the chest wall and may lead to obvious thoracic complications, such as local depression and asymmetry of the bilateral thoracic height.. Decellularized materials have been used for tissue reconstruction in clinical surgeries.. To apply xenogenic decellularized cartilage in costal cartilage defects, porcine-derived auricular and costal cartilage was tested for characterization, cytotoxicity, macrophage response, and tissue regeneration.

    Staining:

    Article Title: Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Article Snippet: .. Cells were then stained with anti-mouse Abs against phycoerythrin (PE)-conjugated CD 54 (60 ng; BioLegend, 116108), allophycocyanin (APC)-conjugated CD86 (6.0 ng; Miltenyi Biotec, 130-102-558), fluorescein isothiocyanate (FITC)-conjugated CD68 (25 ng; Bio-Rad Laboratories, MCA1957F), FITC-conjugated CD163 (200 ng; Bioss, bs-2527R-FITC), FITC-conjugated CD206 (200 ng; Bio-Rad Laboratories, MCA2235F), APC-conjugated M-CSF1 receptor (M-CSF1R) (60 ng; BioLegend, 135510), Alexa Fluor 647-conjugated IL-18Rβ (80 ng; Bioss, bs-2616R-A647), FITC-conjugated integrin α4 (130 ng; Thermo Fisher Scientific, 11-0492-82), or PE-conjugated integrin α9 (0.50 μL; Thermo Fisher Scientific, PA5-46896) for 30 min at 4°C. .. After rinsing with FACS wash buffer followed by centrifugation (500 × g , 5 min, 4°C), 300 μL of PBS was added to the residue followed by staining with propidium iodide (PI) (2 μg/mL; Dojindo Laboratories, P378) to exclude PI-positive dead cells.



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    Image Search Results


    M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while CD163, ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while CD163, ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Flow Cytometry, Standard Deviation

    Liver expression of AKT (a–c), TGFb (d–f), CD163 (g–i), CD86 (j–l), NFkB (m–o), IL-10 (p–r), and Pi3K (s–u) in Wistar rats submitted to alcoholic liver injury.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: Liver expression of AKT (a–c), TGFb (d–f), CD163 (g–i), CD86 (j–l), NFkB (m–o), IL-10 (p–r), and Pi3K (s–u) in Wistar rats submitted to alcoholic liver injury.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Expressing

    Liver expression of AKT (a), TGFb (b), CD163 (c), CD86 (d), NFkB (e), IL-10 (f), and Pi3K (g). All data are presented as mean ± SD. Fluorescence intensity data was generated by Zeiss Zen Lite software for each picture taken (10 images per subject, 5 animals per group, 400× magnification). Statistically significant differences are indicated as follows: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc correction for multiple comparisons.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: Liver expression of AKT (a), TGFb (b), CD163 (c), CD86 (d), NFkB (e), IL-10 (f), and Pi3K (g). All data are presented as mean ± SD. Fluorescence intensity data was generated by Zeiss Zen Lite software for each picture taken (10 images per subject, 5 animals per group, 400× magnification). Statistically significant differences are indicated as follows: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc correction for multiple comparisons.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Expressing, Fluorescence, Generated, Software

    Comparison between M1 (CD86) and M2 (CD163) immunofluorescence labeling in Wistar rats submitted to alcoholic liver injury. All data are presented as mean ± SD. Fluorescence intensity measurements were obtained using Zeiss Zen Lite software, with 10 images captured per subject, 5 animals per group, at 400× magnification. Statistically significant differences are denoted as * p < 0.05 and ** p < 0.005; (ns) indicates no significant difference. Data were analyzed using two-way ANOVA with Sidak’s post hoc correction.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: Comparison between M1 (CD86) and M2 (CD163) immunofluorescence labeling in Wistar rats submitted to alcoholic liver injury. All data are presented as mean ± SD. Fluorescence intensity measurements were obtained using Zeiss Zen Lite software, with 10 images captured per subject, 5 animals per group, at 400× magnification. Statistically significant differences are denoted as * p < 0.05 and ** p < 0.005; (ns) indicates no significant difference. Data were analyzed using two-way ANOVA with Sidak’s post hoc correction.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Comparison, Immunofluorescence, Labeling, Fluorescence, Software